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human bone osteosarcoma cell line 143b  (ATCC)


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    ATCC human bone osteosarcoma cell line 143b
    Human Bone Osteosarcoma Cell Line 143b, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1448 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/143b+human+osteosarcoma+cell+line/143B/us12648992-113-2-31
    Average 97 stars, based on 1448 article reviews
    human bone osteosarcoma cell line 143b - by Bioz Stars, 2026-10
    97/100 stars

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    Related Articles

    In Vitro:

    Article Title: The Glucose and Glutamine Requirements of Cancer and Normal Cells Do Not Distinguish Them, in Contrast to Their Methionine Requirement, Suggesting the Warburg Effect Is Not a Cancer Paradigm.
    Article Snippet: Cell culture.Cell culture.. The 143B human osteosarcoma cell line, the HT1080 human fibrosarcoma cell line, the HCT116 human colon cancer cell line and Hs27 normal human fibroblasts were obtained from the American Type Culture Collection (Manassas, VA, USA).. Green fluorescent protein (GFP) -expressing HCT116 cells, and red fluorescent protein (RFP) -expressing 143B and HT1080 cells, were established as described elsewhere (23).Green fluorescent protein (GFP) -expressing HCT116 cells, and red fluorescent protein (RFP) -expressing 143B and HT1080 cells, were established as described elsewhere (23).

    Article Title: Methionine Restriction Alone Induces T-cell-mediated Immunotherapy of Osteosarcoma in a Syngeneic Mouse Model.
    Article Snippet: Cell lines and culture.Cell lines and culture.. The K7M2 murine osteosarcoma cell line and 143B human osteosarcoma cell line were obtained from the American Type Culture Collection (Manassas, VA, USA).. K7M2 and 143B cells were grown in Dulbecco’s Modified Eagle Medium (DMEM)/Nutrient Mixture F-12 with GlutaMAXTM supplement (GIBCO, Grand Island, NY, USA), 10% fetal bovine serum (GIBCO), and 100 IU/ml penicillin/ streptomycin (GIBCO).K7M2 and 143B cells were grown in Dulbecco’s Modified Eagle Medium (DMEM)/Nutrient Mixture F-12 with GlutaMAXTM supplement (GIBCO, Grand Island, NY, USA), 10% fetal bovine serum (GIBCO), and 100 IU/ml penicillin/ streptomycin (GIBCO).

    Article Title: Methionine Restriction Alone Induces T-cell-mediated Immunotherapy of Osteosarcoma in a Syngeneic Mouse Model
    Article Snippet: Cell lines and culture.Cell lines and culture.. The K7M2 murine osteosarcoma cell line and 143B human osteosarcoma cell line were obtained from the American Type Culture Collection (Manassas, VA, USA).. K7M2 and 143B cells were grown in Dulbecco’s Modified Eagle Medium (DMEM)/Nutrient Mixture F-12 with GlutaMAXTM supplement (GIBCO, Grand Island, NY, USA), 10% fetal bovine serum (GIBCO), and 100 IU/ml penicillin/ streptomycin (GIBCO).K7M2 and 143B cells were grown in Dulbecco’s Modified Eagle Medium (DMEM)/Nutrient Mixture F-12 with GlutaMAXTM supplement (GIBCO, Grand Island, NY, USA), 10% fetal bovine serum (GIBCO), and 100 IU/ml penicillin/ streptomycin (GIBCO).

    Article Title: Methionine Restriction, Not Cysteine Restriction, Is a Cancer-specific Vulnerability.
    Article Snippet: Cell culture.Cell culture.. The HCT-116 human colon-cancer cell line, HT1080 human fibrosarcoma cell line, 143B human osteosarcoma cell line, and Hs27 normal human fibroblasts were obtained from the American Type Culture Collection (Manassas, VA, USA).. Green fluorescent protein-expressing HCT116 cells, and red fluorescent protein-expressing 143B and HT1080 cells were established as described elsewhere (12-14).Green fluorescent protein-expressing HCT116 cells, and red fluorescent protein-expressing 143B and HT1080 cells were established as described elsewhere (12-14).

    Standard Deviation:

    Article Title: The Glucose and Glutamine Requirements of Cancer and Normal Cells Do Not Distinguish Them, in Contrast to Their Methionine Requirement, Suggesting the Warburg Effect Is Not a Cancer Paradigm.
    Article Snippet: Cell culture.Cell culture.. The 143B human osteosarcoma cell line, the HT1080 human fibrosarcoma cell line, the HCT116 human colon cancer cell line and Hs27 normal human fibroblasts were obtained from the American Type Culture Collection (Manassas, VA, USA).. Green fluorescent protein (GFP) -expressing HCT116 cells, and red fluorescent protein (RFP) -expressing 143B and HT1080 cells, were established as described elsewhere (23).Green fluorescent protein (GFP) -expressing HCT116 cells, and red fluorescent protein (RFP) -expressing 143B and HT1080 cells, were established as described elsewhere (23).

    Article Title: Methionine Restriction Alone Induces T-cell-mediated Immunotherapy of Osteosarcoma in a Syngeneic Mouse Model.
    Article Snippet: Cell lines and culture.Cell lines and culture.. The K7M2 murine osteosarcoma cell line and 143B human osteosarcoma cell line were obtained from the American Type Culture Collection (Manassas, VA, USA).. K7M2 and 143B cells were grown in Dulbecco’s Modified Eagle Medium (DMEM)/Nutrient Mixture F-12 with GlutaMAXTM supplement (GIBCO, Grand Island, NY, USA), 10% fetal bovine serum (GIBCO), and 100 IU/ml penicillin/ streptomycin (GIBCO).K7M2 and 143B cells were grown in Dulbecco’s Modified Eagle Medium (DMEM)/Nutrient Mixture F-12 with GlutaMAXTM supplement (GIBCO, Grand Island, NY, USA), 10% fetal bovine serum (GIBCO), and 100 IU/ml penicillin/ streptomycin (GIBCO).

    Article Title: Methionine Restriction Alone Induces T-cell-mediated Immunotherapy of Osteosarcoma in a Syngeneic Mouse Model
    Article Snippet: Cell lines and culture.Cell lines and culture.. The K7M2 murine osteosarcoma cell line and 143B human osteosarcoma cell line were obtained from the American Type Culture Collection (Manassas, VA, USA).. K7M2 and 143B cells were grown in Dulbecco’s Modified Eagle Medium (DMEM)/Nutrient Mixture F-12 with GlutaMAXTM supplement (GIBCO, Grand Island, NY, USA), 10% fetal bovine serum (GIBCO), and 100 IU/ml penicillin/ streptomycin (GIBCO).K7M2 and 143B cells were grown in Dulbecco’s Modified Eagle Medium (DMEM)/Nutrient Mixture F-12 with GlutaMAXTM supplement (GIBCO, Grand Island, NY, USA), 10% fetal bovine serum (GIBCO), and 100 IU/ml penicillin/ streptomycin (GIBCO).

    Article Title: Methionine Restriction, Not Cysteine Restriction, Is a Cancer-specific Vulnerability.
    Article Snippet: Cell culture.Cell culture.. The HCT-116 human colon-cancer cell line, HT1080 human fibrosarcoma cell line, 143B human osteosarcoma cell line, and Hs27 normal human fibroblasts were obtained from the American Type Culture Collection (Manassas, VA, USA).. Green fluorescent protein-expressing HCT116 cells, and red fluorescent protein-expressing 143B and HT1080 cells were established as described elsewhere (12-14).Green fluorescent protein-expressing HCT116 cells, and red fluorescent protein-expressing 143B and HT1080 cells were established as described elsewhere (12-14).

    Concentration Assay:

    Article Title: The Glucose and Glutamine Requirements of Cancer and Normal Cells Do Not Distinguish Them, in Contrast to Their Methionine Requirement, Suggesting the Warburg Effect Is Not a Cancer Paradigm.
    Article Snippet: Cell culture.Cell culture.. The 143B human osteosarcoma cell line, the HT1080 human fibrosarcoma cell line, the HCT116 human colon cancer cell line and Hs27 normal human fibroblasts were obtained from the American Type Culture Collection (Manassas, VA, USA).. Green fluorescent protein (GFP) -expressing HCT116 cells, and red fluorescent protein (RFP) -expressing 143B and HT1080 cells, were established as described elsewhere (23).Green fluorescent protein (GFP) -expressing HCT116 cells, and red fluorescent protein (RFP) -expressing 143B and HT1080 cells, were established as described elsewhere (23).

    Article Title: Methionine Restriction Alone Induces T-cell-mediated Immunotherapy of Osteosarcoma in a Syngeneic Mouse Model.
    Article Snippet: Cell lines and culture.Cell lines and culture.. The K7M2 murine osteosarcoma cell line and 143B human osteosarcoma cell line were obtained from the American Type Culture Collection (Manassas, VA, USA).. K7M2 and 143B cells were grown in Dulbecco’s Modified Eagle Medium (DMEM)/Nutrient Mixture F-12 with GlutaMAXTM supplement (GIBCO, Grand Island, NY, USA), 10% fetal bovine serum (GIBCO), and 100 IU/ml penicillin/ streptomycin (GIBCO).K7M2 and 143B cells were grown in Dulbecco’s Modified Eagle Medium (DMEM)/Nutrient Mixture F-12 with GlutaMAXTM supplement (GIBCO, Grand Island, NY, USA), 10% fetal bovine serum (GIBCO), and 100 IU/ml penicillin/ streptomycin (GIBCO).

    Article Title: Methionine Restriction Alone Induces T-cell-mediated Immunotherapy of Osteosarcoma in a Syngeneic Mouse Model
    Article Snippet: Cell lines and culture.Cell lines and culture.. The K7M2 murine osteosarcoma cell line and 143B human osteosarcoma cell line were obtained from the American Type Culture Collection (Manassas, VA, USA).. K7M2 and 143B cells were grown in Dulbecco’s Modified Eagle Medium (DMEM)/Nutrient Mixture F-12 with GlutaMAXTM supplement (GIBCO, Grand Island, NY, USA), 10% fetal bovine serum (GIBCO), and 100 IU/ml penicillin/ streptomycin (GIBCO).K7M2 and 143B cells were grown in Dulbecco’s Modified Eagle Medium (DMEM)/Nutrient Mixture F-12 with GlutaMAXTM supplement (GIBCO, Grand Island, NY, USA), 10% fetal bovine serum (GIBCO), and 100 IU/ml penicillin/ streptomycin (GIBCO).

    Article Title: Methionine Restriction, Not Cysteine Restriction, Is a Cancer-specific Vulnerability.
    Article Snippet: Cell culture.Cell culture.. The HCT-116 human colon-cancer cell line, HT1080 human fibrosarcoma cell line, 143B human osteosarcoma cell line, and Hs27 normal human fibroblasts were obtained from the American Type Culture Collection (Manassas, VA, USA).. Green fluorescent protein-expressing HCT116 cells, and red fluorescent protein-expressing 143B and HT1080 cells were established as described elsewhere (12-14).Green fluorescent protein-expressing HCT116 cells, and red fluorescent protein-expressing 143B and HT1080 cells were established as described elsewhere (12-14).

    Expressing:

    Article Title: The Glucose and Glutamine Requirements of Cancer and Normal Cells Do Not Distinguish Them, in Contrast to Their Methionine Requirement, Suggesting the Warburg Effect Is Not a Cancer Paradigm.
    Article Snippet: Cell culture.Cell culture.. The 143B human osteosarcoma cell line, the HT1080 human fibrosarcoma cell line, the HCT116 human colon cancer cell line and Hs27 normal human fibroblasts were obtained from the American Type Culture Collection (Manassas, VA, USA).. Green fluorescent protein (GFP) -expressing HCT116 cells, and red fluorescent protein (RFP) -expressing 143B and HT1080 cells, were established as described elsewhere (23).Green fluorescent protein (GFP) -expressing HCT116 cells, and red fluorescent protein (RFP) -expressing 143B and HT1080 cells, were established as described elsewhere (23).

    Article Title: Methionine Restriction Alone Induces T-cell-mediated Immunotherapy of Osteosarcoma in a Syngeneic Mouse Model.
    Article Snippet: Cell lines and culture.Cell lines and culture.. The K7M2 murine osteosarcoma cell line and 143B human osteosarcoma cell line were obtained from the American Type Culture Collection (Manassas, VA, USA).. K7M2 and 143B cells were grown in Dulbecco’s Modified Eagle Medium (DMEM)/Nutrient Mixture F-12 with GlutaMAXTM supplement (GIBCO, Grand Island, NY, USA), 10% fetal bovine serum (GIBCO), and 100 IU/ml penicillin/ streptomycin (GIBCO).K7M2 and 143B cells were grown in Dulbecco’s Modified Eagle Medium (DMEM)/Nutrient Mixture F-12 with GlutaMAXTM supplement (GIBCO, Grand Island, NY, USA), 10% fetal bovine serum (GIBCO), and 100 IU/ml penicillin/ streptomycin (GIBCO).

    Article Title: Methionine Restriction Alone Induces T-cell-mediated Immunotherapy of Osteosarcoma in a Syngeneic Mouse Model
    Article Snippet: Cell lines and culture.Cell lines and culture.. The K7M2 murine osteosarcoma cell line and 143B human osteosarcoma cell line were obtained from the American Type Culture Collection (Manassas, VA, USA).. K7M2 and 143B cells were grown in Dulbecco’s Modified Eagle Medium (DMEM)/Nutrient Mixture F-12 with GlutaMAXTM supplement (GIBCO, Grand Island, NY, USA), 10% fetal bovine serum (GIBCO), and 100 IU/ml penicillin/ streptomycin (GIBCO).K7M2 and 143B cells were grown in Dulbecco’s Modified Eagle Medium (DMEM)/Nutrient Mixture F-12 with GlutaMAXTM supplement (GIBCO, Grand Island, NY, USA), 10% fetal bovine serum (GIBCO), and 100 IU/ml penicillin/ streptomycin (GIBCO).

    Article Title: Methionine Restriction, Not Cysteine Restriction, Is a Cancer-specific Vulnerability.
    Article Snippet: Cell culture.Cell culture.. The HCT-116 human colon-cancer cell line, HT1080 human fibrosarcoma cell line, 143B human osteosarcoma cell line, and Hs27 normal human fibroblasts were obtained from the American Type Culture Collection (Manassas, VA, USA).. Green fluorescent protein-expressing HCT116 cells, and red fluorescent protein-expressing 143B and HT1080 cells were established as described elsewhere (12-14).Green fluorescent protein-expressing HCT116 cells, and red fluorescent protein-expressing 143B and HT1080 cells were established as described elsewhere (12-14).

    Western Blot:

    Article Title: The Glucose and Glutamine Requirements of Cancer and Normal Cells Do Not Distinguish Them, in Contrast to Their Methionine Requirement, Suggesting the Warburg Effect Is Not a Cancer Paradigm.
    Article Snippet: Cell culture.Cell culture.. The 143B human osteosarcoma cell line, the HT1080 human fibrosarcoma cell line, the HCT116 human colon cancer cell line and Hs27 normal human fibroblasts were obtained from the American Type Culture Collection (Manassas, VA, USA).. Green fluorescent protein (GFP) -expressing HCT116 cells, and red fluorescent protein (RFP) -expressing 143B and HT1080 cells, were established as described elsewhere (23).Green fluorescent protein (GFP) -expressing HCT116 cells, and red fluorescent protein (RFP) -expressing 143B and HT1080 cells, were established as described elsewhere (23).

    Article Title: Methionine Restriction Alone Induces T-cell-mediated Immunotherapy of Osteosarcoma in a Syngeneic Mouse Model.
    Article Snippet: Cell lines and culture.Cell lines and culture.. The K7M2 murine osteosarcoma cell line and 143B human osteosarcoma cell line were obtained from the American Type Culture Collection (Manassas, VA, USA).. K7M2 and 143B cells were grown in Dulbecco’s Modified Eagle Medium (DMEM)/Nutrient Mixture F-12 with GlutaMAXTM supplement (GIBCO, Grand Island, NY, USA), 10% fetal bovine serum (GIBCO), and 100 IU/ml penicillin/ streptomycin (GIBCO).K7M2 and 143B cells were grown in Dulbecco’s Modified Eagle Medium (DMEM)/Nutrient Mixture F-12 with GlutaMAXTM supplement (GIBCO, Grand Island, NY, USA), 10% fetal bovine serum (GIBCO), and 100 IU/ml penicillin/ streptomycin (GIBCO).

    Article Title: Methionine Restriction Alone Induces T-cell-mediated Immunotherapy of Osteosarcoma in a Syngeneic Mouse Model
    Article Snippet: Cell lines and culture.Cell lines and culture.. The K7M2 murine osteosarcoma cell line and 143B human osteosarcoma cell line were obtained from the American Type Culture Collection (Manassas, VA, USA).. K7M2 and 143B cells were grown in Dulbecco’s Modified Eagle Medium (DMEM)/Nutrient Mixture F-12 with GlutaMAXTM supplement (GIBCO, Grand Island, NY, USA), 10% fetal bovine serum (GIBCO), and 100 IU/ml penicillin/ streptomycin (GIBCO).K7M2 and 143B cells were grown in Dulbecco’s Modified Eagle Medium (DMEM)/Nutrient Mixture F-12 with GlutaMAXTM supplement (GIBCO, Grand Island, NY, USA), 10% fetal bovine serum (GIBCO), and 100 IU/ml penicillin/ streptomycin (GIBCO).

    Article Title: Methionine Restriction, Not Cysteine Restriction, Is a Cancer-specific Vulnerability.
    Article Snippet: Cell culture.Cell culture.. The HCT-116 human colon-cancer cell line, HT1080 human fibrosarcoma cell line, 143B human osteosarcoma cell line, and Hs27 normal human fibroblasts were obtained from the American Type Culture Collection (Manassas, VA, USA).. Green fluorescent protein-expressing HCT116 cells, and red fluorescent protein-expressing 143B and HT1080 cells were established as described elsewhere (12-14).Green fluorescent protein-expressing HCT116 cells, and red fluorescent protein-expressing 143B and HT1080 cells were established as described elsewhere (12-14).

    Control:

    Article Title: The Glucose and Glutamine Requirements of Cancer and Normal Cells Do Not Distinguish Them, in Contrast to Their Methionine Requirement, Suggesting the Warburg Effect Is Not a Cancer Paradigm.
    Article Snippet: Cell culture.Cell culture.. The 143B human osteosarcoma cell line, the HT1080 human fibrosarcoma cell line, the HCT116 human colon cancer cell line and Hs27 normal human fibroblasts were obtained from the American Type Culture Collection (Manassas, VA, USA).. Green fluorescent protein (GFP) -expressing HCT116 cells, and red fluorescent protein (RFP) -expressing 143B and HT1080 cells, were established as described elsewhere (23).Green fluorescent protein (GFP) -expressing HCT116 cells, and red fluorescent protein (RFP) -expressing 143B and HT1080 cells, were established as described elsewhere (23).

    Article Title: Methionine Restriction Alone Induces T-cell-mediated Immunotherapy of Osteosarcoma in a Syngeneic Mouse Model.
    Article Snippet: Cell lines and culture.Cell lines and culture.. The K7M2 murine osteosarcoma cell line and 143B human osteosarcoma cell line were obtained from the American Type Culture Collection (Manassas, VA, USA).. K7M2 and 143B cells were grown in Dulbecco’s Modified Eagle Medium (DMEM)/Nutrient Mixture F-12 with GlutaMAXTM supplement (GIBCO, Grand Island, NY, USA), 10% fetal bovine serum (GIBCO), and 100 IU/ml penicillin/ streptomycin (GIBCO).K7M2 and 143B cells were grown in Dulbecco’s Modified Eagle Medium (DMEM)/Nutrient Mixture F-12 with GlutaMAXTM supplement (GIBCO, Grand Island, NY, USA), 10% fetal bovine serum (GIBCO), and 100 IU/ml penicillin/ streptomycin (GIBCO).

    Article Title: Methionine Restriction Alone Induces T-cell-mediated Immunotherapy of Osteosarcoma in a Syngeneic Mouse Model
    Article Snippet: Cell lines and culture.Cell lines and culture.. The K7M2 murine osteosarcoma cell line and 143B human osteosarcoma cell line were obtained from the American Type Culture Collection (Manassas, VA, USA).. K7M2 and 143B cells were grown in Dulbecco’s Modified Eagle Medium (DMEM)/Nutrient Mixture F-12 with GlutaMAXTM supplement (GIBCO, Grand Island, NY, USA), 10% fetal bovine serum (GIBCO), and 100 IU/ml penicillin/ streptomycin (GIBCO).K7M2 and 143B cells were grown in Dulbecco’s Modified Eagle Medium (DMEM)/Nutrient Mixture F-12 with GlutaMAXTM supplement (GIBCO, Grand Island, NY, USA), 10% fetal bovine serum (GIBCO), and 100 IU/ml penicillin/ streptomycin (GIBCO).

    Article Title: Methionine Restriction, Not Cysteine Restriction, Is a Cancer-specific Vulnerability.
    Article Snippet: Cell culture.Cell culture.. The HCT-116 human colon-cancer cell line, HT1080 human fibrosarcoma cell line, 143B human osteosarcoma cell line, and Hs27 normal human fibroblasts were obtained from the American Type Culture Collection (Manassas, VA, USA).. Green fluorescent protein-expressing HCT116 cells, and red fluorescent protein-expressing 143B and HT1080 cells were established as described elsewhere (12-14).Green fluorescent protein-expressing HCT116 cells, and red fluorescent protein-expressing 143B and HT1080 cells were established as described elsewhere (12-14).



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    Image Search Results


    Antitumor effect of organometallic compounds in osteosarcoma cell lines. Cell viability (WST-1 assay) in 2D cultures measured after the treatment of 143B Par (A), OS833 Par (B), 143B CisPt-R (C), and OS833 CisPt-R (D) with increasing concentrations of free or encapsulated drugs for 72 h. The effect of equivalent amounts of empty nanoparticles in each cell line is shown. IC 50 values for each treatment are indicated. Error bars represent the standard deviation of three independent experiments. Asterisks indicate statistically significant differences between cisplatin and Ru3 (black) or LIP-Ru3 (salmon) (**** p < 0.0001; two-way ANOVA Tukey's test). (E and F) Analysis of DNA damage (formation of −H2AX foci) in 143B CisPt-R cells treated with the IC 75 of CisPt (8.4 µM), Ru3 (0.9 µM), LIP-Ru3 (0.8 µM), or the equivalent amount of empty nanoparticles (LIP-Empty) for 24 hours. Representative images of immunofluorescence staining (scale bar = 25 µm) (E) and quantification of the formation of gH2AX foci in >100 cells (F) are shown. Asterisks indicate statistically significant differences (** p < 0.01; *** p < 0.001; One-way ANOVA Tukey's test). (G) Cell cycle distribution of 143B CisPt-R cells treated with the IC 50 of CisPt (5 µM), Ru3 (0.5 µM), LIP-Ru3 (0.5 µM), or the equivalent amount of empty nanoparticles (LIP-Empty) for 48 hours. The normalized percentage of cells in each cell cycle phase (mean ± standard deviation of three independent experiments) is shown. Asterisks indicate statistically significant differences in G 0 /G 1 phase (blue) between LIP-empty control and Ru3-formulations and in the G 2 /M phase (pink) between LIP-empty and CisPt (**** p < 0.0001; two-way ANOVA Tukey's test).

    Journal: Drug Delivery

    Article Title: Liposome-mediated delivery of a ruthenium-based metallodrug to overcome cisplatin resistance in osteosarcoma

    doi: 10.1080/10717544.2026.2671485

    Figure Lengend Snippet: Antitumor effect of organometallic compounds in osteosarcoma cell lines. Cell viability (WST-1 assay) in 2D cultures measured after the treatment of 143B Par (A), OS833 Par (B), 143B CisPt-R (C), and OS833 CisPt-R (D) with increasing concentrations of free or encapsulated drugs for 72 h. The effect of equivalent amounts of empty nanoparticles in each cell line is shown. IC 50 values for each treatment are indicated. Error bars represent the standard deviation of three independent experiments. Asterisks indicate statistically significant differences between cisplatin and Ru3 (black) or LIP-Ru3 (salmon) (**** p < 0.0001; two-way ANOVA Tukey's test). (E and F) Analysis of DNA damage (formation of −H2AX foci) in 143B CisPt-R cells treated with the IC 75 of CisPt (8.4 µM), Ru3 (0.9 µM), LIP-Ru3 (0.8 µM), or the equivalent amount of empty nanoparticles (LIP-Empty) for 24 hours. Representative images of immunofluorescence staining (scale bar = 25 µm) (E) and quantification of the formation of gH2AX foci in >100 cells (F) are shown. Asterisks indicate statistically significant differences (** p < 0.01; *** p < 0.001; One-way ANOVA Tukey's test). (G) Cell cycle distribution of 143B CisPt-R cells treated with the IC 50 of CisPt (5 µM), Ru3 (0.5 µM), LIP-Ru3 (0.5 µM), or the equivalent amount of empty nanoparticles (LIP-Empty) for 48 hours. The normalized percentage of cells in each cell cycle phase (mean ± standard deviation of three independent experiments) is shown. Asterisks indicate statistically significant differences in G 0 /G 1 phase (blue) between LIP-empty control and Ru3-formulations and in the G 2 /M phase (pink) between LIP-empty and CisPt (**** p < 0.0001; two-way ANOVA Tukey's test).

    Article Snippet: Human osteosarcoma cell line 143B (CRL-8303) was obtained from the ATCC repository (Manassas, VA, USA).

    Techniques: WST-1 Assay, Standard Deviation, Immunofluorescence, Staining, Control

    Cytotoxic effect of organometallic compounds in 3D parental spheroids. Spheroid viability (CTG 3D assay) was measured after the treatment of 143B Par (A and B) and OS833 Par (C and D) spheroids with increasing concentrations of free and encapsulated drugs for 96 h. The effect of equivalent amounts of empty nanoparticles is shown. Representative images of 143B Par (A) and OS833 Par (C) spheroids treated with increasing concentrations of each compound are shown (scale bars = 250 µm). Likewise, cell survival curves and IC50 values (µM) for each condition in 143B Par (B) and OS833 Par (D) spheroids are presented as the mean ± the standard deviation of at least eight biological replicates. Asterisks indicate statistically significant differences between cisplatin and Ru3 (black) or LIP-Ru3 (salmon) (**** p < 0.0001; two-way ANOVA Tukey's test).

    Journal: Drug Delivery

    Article Title: Liposome-mediated delivery of a ruthenium-based metallodrug to overcome cisplatin resistance in osteosarcoma

    doi: 10.1080/10717544.2026.2671485

    Figure Lengend Snippet: Cytotoxic effect of organometallic compounds in 3D parental spheroids. Spheroid viability (CTG 3D assay) was measured after the treatment of 143B Par (A and B) and OS833 Par (C and D) spheroids with increasing concentrations of free and encapsulated drugs for 96 h. The effect of equivalent amounts of empty nanoparticles is shown. Representative images of 143B Par (A) and OS833 Par (C) spheroids treated with increasing concentrations of each compound are shown (scale bars = 250 µm). Likewise, cell survival curves and IC50 values (µM) for each condition in 143B Par (B) and OS833 Par (D) spheroids are presented as the mean ± the standard deviation of at least eight biological replicates. Asterisks indicate statistically significant differences between cisplatin and Ru3 (black) or LIP-Ru3 (salmon) (**** p < 0.0001; two-way ANOVA Tukey's test).

    Article Snippet: Human osteosarcoma cell line 143B (CRL-8303) was obtained from the ATCC repository (Manassas, VA, USA).

    Techniques: Standard Deviation

    Cytotoxic effect of organometallic compounds in 3D cisplatin-resistant spheroids. Spheroid viability (CTG 3D assay) was measured after the treatment of 143B CisPt-R (A and B) and OS833 CisPt-R (C and D) spheroids with increasing concentrations of free and encapsulated drugs for 96 h. The effect of equivalent amounts of empty nanoparticles is shown. Representative images of 143B CisPt-R (A) and OS833 CisPt-R (C) spheroids treated with increasing concentrations of each compound are shown (scale bars = 250 µm). Cell survival curves and IC50 values (µM) for each condition in 143B CisPt-R (B) and OS833 CisPt-R (D) are presented as the mean ± standard deviation of at least eight biological replicates. Asterisks indicate statistically significant differences between cisplatin and Ru3 (black) or LIP-Ru3 (salmon) (* p < 0.05; ** p < 0.01; **** p < 0.0001; two-way ANOVA Tukey's test).

    Journal: Drug Delivery

    Article Title: Liposome-mediated delivery of a ruthenium-based metallodrug to overcome cisplatin resistance in osteosarcoma

    doi: 10.1080/10717544.2026.2671485

    Figure Lengend Snippet: Cytotoxic effect of organometallic compounds in 3D cisplatin-resistant spheroids. Spheroid viability (CTG 3D assay) was measured after the treatment of 143B CisPt-R (A and B) and OS833 CisPt-R (C and D) spheroids with increasing concentrations of free and encapsulated drugs for 96 h. The effect of equivalent amounts of empty nanoparticles is shown. Representative images of 143B CisPt-R (A) and OS833 CisPt-R (C) spheroids treated with increasing concentrations of each compound are shown (scale bars = 250 µm). Cell survival curves and IC50 values (µM) for each condition in 143B CisPt-R (B) and OS833 CisPt-R (D) are presented as the mean ± standard deviation of at least eight biological replicates. Asterisks indicate statistically significant differences between cisplatin and Ru3 (black) or LIP-Ru3 (salmon) (* p < 0.05; ** p < 0.01; **** p < 0.0001; two-way ANOVA Tukey's test).

    Article Snippet: Human osteosarcoma cell line 143B (CRL-8303) was obtained from the ATCC repository (Manassas, VA, USA).

    Techniques: Standard Deviation

    Effect of free and nano-encapsulated Ru3 in CSC-enriched 3D osteosarcoma cell cultures. 143B Par cells were plated at low density in tumorsphere medium and left to form tumorspheres for 9 days before being treated for 96 h with increasing concentrations of Ru3, LIP-Ru3, and cisplatin. Treatments with the same amount of empty nanoparticles (LIP-Empty) were also included. Representative images (scale bars = 250 μm) (A), quantification of the number of tumorspheres remaining after the treatment (B), and cell viability (WST-1 assay) (C) are presented. Data is presented as the mean ± the standard deviation of independent replicates. In panel B, black asterisks indicate statistically significant differences between controls and the indicated conditions in each treatment. In panel C, asterisks indicate statistically significant differences between cisplatin and Ru3 (black) or LIP-Ru3 (salmon); (** p < 0.01; *** p < 0.001; one-way (panel B) or two-way (panel C) ANOVA Tukey's test).

    Journal: Drug Delivery

    Article Title: Liposome-mediated delivery of a ruthenium-based metallodrug to overcome cisplatin resistance in osteosarcoma

    doi: 10.1080/10717544.2026.2671485

    Figure Lengend Snippet: Effect of free and nano-encapsulated Ru3 in CSC-enriched 3D osteosarcoma cell cultures. 143B Par cells were plated at low density in tumorsphere medium and left to form tumorspheres for 9 days before being treated for 96 h with increasing concentrations of Ru3, LIP-Ru3, and cisplatin. Treatments with the same amount of empty nanoparticles (LIP-Empty) were also included. Representative images (scale bars = 250 μm) (A), quantification of the number of tumorspheres remaining after the treatment (B), and cell viability (WST-1 assay) (C) are presented. Data is presented as the mean ± the standard deviation of independent replicates. In panel B, black asterisks indicate statistically significant differences between controls and the indicated conditions in each treatment. In panel C, asterisks indicate statistically significant differences between cisplatin and Ru3 (black) or LIP-Ru3 (salmon); (** p < 0.01; *** p < 0.001; one-way (panel B) or two-way (panel C) ANOVA Tukey's test).

    Article Snippet: Human osteosarcoma cell line 143B (CRL-8303) was obtained from the ATCC repository (Manassas, VA, USA).

    Techniques: WST-1 Assay, Standard Deviation

    In vivo antitumor effect of free and nanoencapsulated metallic compounds. Established 143B CisPt-R xenografts were randomly assigned to six different groups ( n = 5 per group) and treated i.v. with vehicle (PBS, control), empty liposomes (LIP-Empty), free Ru3 (4 mg/kg, Ru3), ruthenium-loaded liposomes (4 mg/kg, LIP-Ru3), RAPTA-C (4 mg/kg), and cisplatin (4 mg/kg) once a week for 3 weeks (cumulative dose of 12 mg/kg). (A) Curves representing the mean relative tumor volume of 143B CisPt-R xenografts during the treatments. Drug efficacy expressed as the percentage of TGI is indicated. (B) Average tumor weight (left panel) and images of the tumors (right panel) at the end of the experiment (day 20 after the start of the treatment). (C) Change in the body weights of mice during the treatments. Error bars represent the SEM and asterisks indicate statistically significant differences between groups (* p < 0.05; **** p < 0.0001, two-way ANOVA Tukey's test).

    Journal: Drug Delivery

    Article Title: Liposome-mediated delivery of a ruthenium-based metallodrug to overcome cisplatin resistance in osteosarcoma

    doi: 10.1080/10717544.2026.2671485

    Figure Lengend Snippet: In vivo antitumor effect of free and nanoencapsulated metallic compounds. Established 143B CisPt-R xenografts were randomly assigned to six different groups ( n = 5 per group) and treated i.v. with vehicle (PBS, control), empty liposomes (LIP-Empty), free Ru3 (4 mg/kg, Ru3), ruthenium-loaded liposomes (4 mg/kg, LIP-Ru3), RAPTA-C (4 mg/kg), and cisplatin (4 mg/kg) once a week for 3 weeks (cumulative dose of 12 mg/kg). (A) Curves representing the mean relative tumor volume of 143B CisPt-R xenografts during the treatments. Drug efficacy expressed as the percentage of TGI is indicated. (B) Average tumor weight (left panel) and images of the tumors (right panel) at the end of the experiment (day 20 after the start of the treatment). (C) Change in the body weights of mice during the treatments. Error bars represent the SEM and asterisks indicate statistically significant differences between groups (* p < 0.05; **** p < 0.0001, two-way ANOVA Tukey's test).

    Article Snippet: Human osteosarcoma cell line 143B (CRL-8303) was obtained from the ATCC repository (Manassas, VA, USA).

    Techniques: In Vivo, Control, Liposomes

    Identification of active compounds and target prediction in YHD. (A) Venn diagram of the target of YHD and the target of osteosarcoma. (B – D) Gene Ontology (GO) enrichment analysis results. (E, F) Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis results. (G) The component-target-pathway-disease network implicated in the mechanism of YHD in osteosarcoma treatment. The triangles represent osteosarcoma, the diamonds represent pathways, the circles represent key genes, and the squares represent the active ingredients of YHD. (H) Heatmap of molecular docking score. A binding energy heatmap with a bluer color indicates a more stable binding. (I) Molecular docking visualization between the active components of YHD and key targets.

    Journal: Genes & Diseases

    Article Title: Network pharmacology reveals that Yanghe Decoction inhibits osteosarcoma progression via ROS-induced mitochondrial dysfunction and enhances cisplatin sensitivity

    doi: 10.1016/j.gendis.2025.101862

    Figure Lengend Snippet: Identification of active compounds and target prediction in YHD. (A) Venn diagram of the target of YHD and the target of osteosarcoma. (B – D) Gene Ontology (GO) enrichment analysis results. (E, F) Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis results. (G) The component-target-pathway-disease network implicated in the mechanism of YHD in osteosarcoma treatment. The triangles represent osteosarcoma, the diamonds represent pathways, the circles represent key genes, and the squares represent the active ingredients of YHD. (H) Heatmap of molecular docking score. A binding energy heatmap with a bluer color indicates a more stable binding. (I) Molecular docking visualization between the active components of YHD and key targets.

    Article Snippet: Human osteosarcoma cell lines (HOS, 143B), human bone marrow stromal cells (HS-5), human proximal renal tubular epithelial cells (HK-2), and human normal liver cells (LO2) were all purchased from the American Type Culture Collection (ATCC).

    Techniques: Binding Assay

    YHD selectively inhibits osteosarcoma (OS) cells without affecting the viability or apoptosis of normal human cells. (A, B) CCK8 assay detected the effect of YHD on the viability of OS cells at 24 h and 48 h. (C, D) Colony formation assay detected the effect of YHD on the colony-forming ability of OS cells. (E – G) Flow cytometry was used to detect the effect of YHD on the cell cycle of OS cells. (H – J) Western blot analysis detected the effect of YHD on the levels of proliferation-related proteins in OS cells. Data were presented as mean ± standard deviation ( n = 3). ∗ p < 0.05 and ∗∗ p < 0.01 versus the blank group.

    Journal: Genes & Diseases

    Article Title: Network pharmacology reveals that Yanghe Decoction inhibits osteosarcoma progression via ROS-induced mitochondrial dysfunction and enhances cisplatin sensitivity

    doi: 10.1016/j.gendis.2025.101862

    Figure Lengend Snippet: YHD selectively inhibits osteosarcoma (OS) cells without affecting the viability or apoptosis of normal human cells. (A, B) CCK8 assay detected the effect of YHD on the viability of OS cells at 24 h and 48 h. (C, D) Colony formation assay detected the effect of YHD on the colony-forming ability of OS cells. (E – G) Flow cytometry was used to detect the effect of YHD on the cell cycle of OS cells. (H – J) Western blot analysis detected the effect of YHD on the levels of proliferation-related proteins in OS cells. Data were presented as mean ± standard deviation ( n = 3). ∗ p < 0.05 and ∗∗ p < 0.01 versus the blank group.

    Article Snippet: Human osteosarcoma cell lines (HOS, 143B), human bone marrow stromal cells (HS-5), human proximal renal tubular epithelial cells (HK-2), and human normal liver cells (LO2) were all purchased from the American Type Culture Collection (ATCC).

    Techniques: CCK-8 Assay, Colony Assay, Flow Cytometry, Western Blot, Standard Deviation

    YHD can inhibit the migration and invasion of osteosarcoma (OS) cells, and promote their apoptosis. (A, B) Scratch healing assay showed that YHD inhibited the migration of OS cells. (C, D) Transwell assay showed that YHD inhibited the invasion of OS cells. (E – G) Western blot analysis detected the effect of YHD on the levels of proteins related to migration and invasion in OS cells. (H–K) Flow cytometry was used to detect the effect of YHD on apoptosis in OS cells. (L – N) Western blot analysis detected the effect of YHD on the levels of apoptosis-related proteins in OS cells. Data were presented as mean ± standard deviation ( n = 3). ∗ p < 0.05 and ∗∗ p < 0.01 versus the blank group.

    Journal: Genes & Diseases

    Article Title: Network pharmacology reveals that Yanghe Decoction inhibits osteosarcoma progression via ROS-induced mitochondrial dysfunction and enhances cisplatin sensitivity

    doi: 10.1016/j.gendis.2025.101862

    Figure Lengend Snippet: YHD can inhibit the migration and invasion of osteosarcoma (OS) cells, and promote their apoptosis. (A, B) Scratch healing assay showed that YHD inhibited the migration of OS cells. (C, D) Transwell assay showed that YHD inhibited the invasion of OS cells. (E – G) Western blot analysis detected the effect of YHD on the levels of proteins related to migration and invasion in OS cells. (H–K) Flow cytometry was used to detect the effect of YHD on apoptosis in OS cells. (L – N) Western blot analysis detected the effect of YHD on the levels of apoptosis-related proteins in OS cells. Data were presented as mean ± standard deviation ( n = 3). ∗ p < 0.05 and ∗∗ p < 0.01 versus the blank group.

    Article Snippet: Human osteosarcoma cell lines (HOS, 143B), human bone marrow stromal cells (HS-5), human proximal renal tubular epithelial cells (HK-2), and human normal liver cells (LO2) were all purchased from the American Type Culture Collection (ATCC).

    Techniques: Migration, Transwell Assay, Western Blot, Flow Cytometry, Standard Deviation

    YHD induces osteosarcoma (OS) cell death by increasing ROS levels. (A) The effect of YHD on ROS levels in OS cells was detected using the DCFH probe method. (B) After N-acetylcysteine (NAC) treatment, the effect of YHD on ROS levels in OS cells was detected using the DCFH probe method. (C) After NAC treatment, CCK8 assay detected the effect of YHD on the viability of OS cells at 24 h and 48 h. (D, E) After NAC treatment, scratch healing assay showed that YHD inhibited the migration of OS cells. (F, G) After NAC treatment, Transwell assay showed that YHD inhibited the invasion of OS cells. (H, I) After NAC treatment, flow cytometry was used to detect the effect of YHD on the apoptosis of OS cells. Data were presented as mean ± standard deviation ( n = 3). ∗ p < 0.05 and ∗∗ p < 0.01 versus the blank group.

    Journal: Genes & Diseases

    Article Title: Network pharmacology reveals that Yanghe Decoction inhibits osteosarcoma progression via ROS-induced mitochondrial dysfunction and enhances cisplatin sensitivity

    doi: 10.1016/j.gendis.2025.101862

    Figure Lengend Snippet: YHD induces osteosarcoma (OS) cell death by increasing ROS levels. (A) The effect of YHD on ROS levels in OS cells was detected using the DCFH probe method. (B) After N-acetylcysteine (NAC) treatment, the effect of YHD on ROS levels in OS cells was detected using the DCFH probe method. (C) After NAC treatment, CCK8 assay detected the effect of YHD on the viability of OS cells at 24 h and 48 h. (D, E) After NAC treatment, scratch healing assay showed that YHD inhibited the migration of OS cells. (F, G) After NAC treatment, Transwell assay showed that YHD inhibited the invasion of OS cells. (H, I) After NAC treatment, flow cytometry was used to detect the effect of YHD on the apoptosis of OS cells. Data were presented as mean ± standard deviation ( n = 3). ∗ p < 0.05 and ∗∗ p < 0.01 versus the blank group.

    Article Snippet: Human osteosarcoma cell lines (HOS, 143B), human bone marrow stromal cells (HS-5), human proximal renal tubular epithelial cells (HK-2), and human normal liver cells (LO2) were all purchased from the American Type Culture Collection (ATCC).

    Techniques: CCK-8 Assay, Migration, Transwell Assay, Flow Cytometry, Standard Deviation

    YHD can induce mitochondrial dysfunction in osteosarcoma (OS) cells. (A) Real-time quantitative PCR was used to measure mitochondrial DNA (mtDNA) levels. (B, C) Western blot analysis detected the effect of YHD on the expression levels of proteins related to mitochondrial biogenesis in OS cells. (D, E) JC-1 staining detected the effect of YHD on the mitochondrial membrane potential in OS cells. (F, G) MitoSOX staining detected the effect of YHD on mitochondrial ROS levels in OS cells. (H) Seahorse XFe24 analyzer measured the effect of YHD on the oxygen consumption rate (OCR) in OS cells. (I) The effect of YHD on ATP content in OS cells. Data were presented as mean ± standard deviation ( n = 3). ∗ p < 0.05 and ∗∗ p < 0.01 versus the blank group.

    Journal: Genes & Diseases

    Article Title: Network pharmacology reveals that Yanghe Decoction inhibits osteosarcoma progression via ROS-induced mitochondrial dysfunction and enhances cisplatin sensitivity

    doi: 10.1016/j.gendis.2025.101862

    Figure Lengend Snippet: YHD can induce mitochondrial dysfunction in osteosarcoma (OS) cells. (A) Real-time quantitative PCR was used to measure mitochondrial DNA (mtDNA) levels. (B, C) Western blot analysis detected the effect of YHD on the expression levels of proteins related to mitochondrial biogenesis in OS cells. (D, E) JC-1 staining detected the effect of YHD on the mitochondrial membrane potential in OS cells. (F, G) MitoSOX staining detected the effect of YHD on mitochondrial ROS levels in OS cells. (H) Seahorse XFe24 analyzer measured the effect of YHD on the oxygen consumption rate (OCR) in OS cells. (I) The effect of YHD on ATP content in OS cells. Data were presented as mean ± standard deviation ( n = 3). ∗ p < 0.05 and ∗∗ p < 0.01 versus the blank group.

    Article Snippet: Human osteosarcoma cell lines (HOS, 143B), human bone marrow stromal cells (HS-5), human proximal renal tubular epithelial cells (HK-2), and human normal liver cells (LO2) were all purchased from the American Type Culture Collection (ATCC).

    Techniques: Real-time Polymerase Chain Reaction, Western Blot, Expressing, Staining, Membrane, Standard Deviation

    YHD exerts anti-tumor effects on osteosarcoma (OS) cells through the PI3K/AKT and p38 signaling pathways. (A) Principal component analysis revealed a clear distinction in gene expression profiles between the control and YHD groups. (B) Volcano plot identified 3495 differentially expressed genes in the YHD group. (C) Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses. (D – G) Gene Set Enrichment Analysis (GSEA) of control and YHD groups. (H, I) Western blot analysis detected the effect of YHD on proteins related to the PI3K/AKT and MAPK pathways in OS cells. (J, K) After the addition of a PI3K activator and a P38 inhibitor, scratch healing assay showed that YHD inhibited the migration of OS cells. (L, M) After the addition of a PI3K activator and a P38 inhibitor, JC-1 staining detected the effect of YHD on the mitochondrial membrane potential in OS cells. Data were presented as mean ± standard deviation ( n = 3). ∗ p < 0.05 and ∗∗ p < 0.01 versus the blank group.

    Journal: Genes & Diseases

    Article Title: Network pharmacology reveals that Yanghe Decoction inhibits osteosarcoma progression via ROS-induced mitochondrial dysfunction and enhances cisplatin sensitivity

    doi: 10.1016/j.gendis.2025.101862

    Figure Lengend Snippet: YHD exerts anti-tumor effects on osteosarcoma (OS) cells through the PI3K/AKT and p38 signaling pathways. (A) Principal component analysis revealed a clear distinction in gene expression profiles between the control and YHD groups. (B) Volcano plot identified 3495 differentially expressed genes in the YHD group. (C) Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses. (D – G) Gene Set Enrichment Analysis (GSEA) of control and YHD groups. (H, I) Western blot analysis detected the effect of YHD on proteins related to the PI3K/AKT and MAPK pathways in OS cells. (J, K) After the addition of a PI3K activator and a P38 inhibitor, scratch healing assay showed that YHD inhibited the migration of OS cells. (L, M) After the addition of a PI3K activator and a P38 inhibitor, JC-1 staining detected the effect of YHD on the mitochondrial membrane potential in OS cells. Data were presented as mean ± standard deviation ( n = 3). ∗ p < 0.05 and ∗∗ p < 0.01 versus the blank group.

    Article Snippet: Human osteosarcoma cell lines (HOS, 143B), human bone marrow stromal cells (HS-5), human proximal renal tubular epithelial cells (HK-2), and human normal liver cells (LO2) were all purchased from the American Type Culture Collection (ATCC).

    Techniques: Protein-Protein interactions, Gene Expression, Control, Western Blot, Migration, Staining, Membrane, Standard Deviation